Contact an Account ManagerBenzodiazepine midazolam and metabolite from plasma
Summary
Type: HPLC
Application #: 118470
Catalog#: 760081.40 - MN HPLC Column, Nucleodur C18 Gravity, 110A, 3um, 4x125mm
Detection: UV, DAD
Conditions: Eluents:
127 ml KH2PO4 buffer (9,1 g in 1000 ml H2O)
309 ml Na2HPO4 buffer (11,9 g in 1000 ml H2O)
852 ml methanol + 0,15 g octansulfonic acid = pH 5,56
Injection: 10 μl
Flow rate: 0,7 ml/min
Authors: Richter
Source: Universität Erlangen, Institut für Anästhesie